|
Statistical analysis
of T-RFLP data that include objective methods for
(a) determining a
baseline so that “true” peaks in electropherograms
can be identified,
(b) a means to compare
electropherograms and bin fragments of similar
size,
(c) clustering algorithms
that can be used to identify communities that
are similar to one another, and
(d) a means to select
samples that are representative of a cluster that
can be used to
construct 16S rRNA gene
clone libraries.
INSTRUCTIONS
- The
programs are
written in R, perl and SAS.
- R is a
language
and environment for
statistical computing and graphics. It is also free. Download R and
install. Perl is also free. Download Perl and install.
- You will
need to have
access to the SAS. SAS is a commonly used commercial statistical
software package. If you do not have SAS, you can learn more
about purchasing this software by clicking
here.
- Download,
read and follow the instructions in the Manual
(word file),
before
running these programs.
R
Code Filter
and Binning (FilteringandBinning.txt)
Cover
Program
(CoverProgram.txt)
SAS
Macro
(ClusteringMacroSingleuse.sas)
EXAMPLE DATA
|
