Freezing Tolerance and Cold Acclimation of Arctic and Subarctic Cushion Plants
The cushion plant, Silena Acaulis, is an arctic tundra species of circumpolar distribution.
It also occurs in alpine environments. This photograph was taken at Ny Ålesund, Svalbard, Norway (78° 55' N lat.)
in mid-August. Episodic frost can occur throughout the short growing season
these high latitude environments. Internal temperatures of the cushion can exceed 10°C above ambient temperatures.
Note that this individual has been colonized by Salix polaris (rounded yellow-green leaves).
With:
Olavi Junttila, Professor, Department of Plant Physiology and Microbiology
Institute of Biology and Geology, University of Tromsø, Tromsø, Norway
Funded by Norwegian Research Council and the University of Tromsø Phytotron, Jarle Nilsen, Director.
Selected References
Junttila, O. and R. Robberecht. 1993.The Influence of Season and Phenology on Freezing Tolerance
in Silene acaulis L., a Subarctic and Arctic Cushion Plant
of Circumpolar Distribution. Annals of Botany 71:423-426.
Abstract Changes in the freezing tolerance for Silene acaulis L.,
a subarctic and arctic species of circumpolar distribution, were
examined to understand the extent of cold hardening and dehardening
that occurs seasonally and with changes in plant phenology. Shoots
of whole plants collected on a mountain ridge near Tromsø,
Norway (69° N, 700 m above sea level) were frozen under
controlled conditions at cooling rates of 3 to 4°C h-1.
The extent of freezing-induced injury was examined both by chlorophyll
fluorescence and by visual inspection with a microscope. A freezing
tolerance level of -30°C was observed in mid-winter, based
on a 50% lethal point for freezing injury. Loss of cold hardiness
was substantial in mid-summer, with freezing tolerances of -8.5
to -9°C observed in mid-July. Plants still covered by snow
in mid-July had a freezing tolerance of -12.5 to -13°C.
The maintenance of a basic level of freezing tolerance throughout
the summer may be adaptive in the northern latitude-regions because
of the occurrence of episodic frosts during the growing season.
Robberecht, R. and O. Junttila. 1992. The freezing response of an arctic cushion plant, Saxifraga caespitosa L.: Acclimation, freezing tolerance and ice nucleation. Annals of Botany 70:129-135.
Abstract Cold hardiness in actively growing plants of Saxifraga caespitosa
L. , an arctic and subarctic cushion plant, was examined. Plants
collected from subarctic and arctic sites were cultivated in a
phytotron at temperatures of 3, 9, 12 and 21°C under a 24-h
photoperiod, and examined for freezing tolerance using controlled
freezing at a cooling rate of 3-4°C either in air or in moist
sand. Post-freezing injury was assessed by visual inspection
and with chlorophyll fluorescence, which appeared to be well suited
for the evaluation of injury in Saxifraga leaves. Freezing
of excised leaves in moist sand distinguished well among the various
treatments, but the differences were partly masked by significant
supercooling when the tissue was frozen in air. Excised leaves,
meristems, stem tissue and flowers supercooled to -9 to -15°C,
but in rosettes and in intact plants ice nucleation was initiated
at -4 to -7°C. The arctic plants tended to be more cold
hardy than the subarctic plants, but in plants from both locations
cold hardiness increased significantly with decreasing growth
temperature. Plants grown at 12°C or less developed resistance
to freezing, and excised leaves of arctic Saxifraga grown
at 3°C survived temperatures down to about -20°C. Exposure
to -3°C temperature for up to 5 d did not significantly enhance
the hardiness obtained at 3°C. When whole plants of arctic
Saxifraga were frozen, with roots protected from freezing,
they survived -15°C and -25°C when cultivated at 12
and 3°C, respectively, although a high percentage of the
leaves were killed. The basal level of freezing tolerance maintained
in these plants throughout periods of active growth may have adaptive
significance in subarctic and arctic environments.
Hagen, S. and G.G. Spomer. 1989. Soil temperature control of growth form in the arctic-alpine cushion plant Silene acualis. Arctic and Alpine Research 21:163-168.
Warren Wilson, J. 1957. Observations on the temperatures of arctic plants and their environmnent. Journal of Ecology 45:499-531.