of T-RFLP data that include objective methods for
(a) determining a
baseline so that “true” peaks in electropherograms
can be identified,
(b) a means to compare
electropherograms and bin fragments of similar
(c) clustering algorithms
that can be used to identify communities that
are similar to one another, and
(d) a means to select
samples that are representative of a cluster that
can be used to
construct 16S rRNA gene
written in R, perl and SAS.
- R is a
and environment for
statistical computing and graphics. It is also free. Download R and
install. Perl is also free. Download Perl and install.
- You will
need to have
access to the SAS. SAS is a commonly used commercial statistical
software package. If you do not have SAS, you can learn more
about purchasing this software by clicking
read and follow the instructions in the Manual
running these programs.
- For Mac
users download the following zipped
directory. Unzip and follow the instructions.
and Binning (FilteringandBinning.txt)