Dual Reporter System for in situ Detection of Plasmid Transfer in Aerobic and Anaerobic Conditions

JE Krol, LM Rogers, SM Krone, and EM Top


We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.