Alteration
of foliar flavonoid composition induced by enhanced UV-B radiation in Pinus
ponderosa, Quercus rubra, Pseudotsuga menziesii and Populus
trichocarpa. |
Warren1, J.M.,
J.H. Bassman1, D.S. Mattinson2, J.K. Fellman2,
G.E. Edwards3, and R. Robberecht4. 2000.
North American Forest Biology Workshop in Merida, Mexico. July 16-20, 2000. |
HPLC analyses of foliage from several common north temperate tree species illustrate the species-specific effects of UV-B radiation on both quantity and composition of foliar flavonoids. Pinus ponderosa, Quercus rubra and Pseudotsuga menziesii were field-grown under ambient (1X) and elevated (2X) biologically effective UV-B radiation (UV-BBE) for Pullman, WA, USA. A computer-controlled modulated lamp system was used to supply the supplemental UV-B radiation. Additionally, Populus tricocarpa was grown in a glasshouse under 0X, 1X and 2X UV-BBE treatments. Foliage was harvested seasonally over a three-year period, extracted, purified and applied to a μBondapak/C18 column HPLC system sampling at 254 nm. Twelve standards were applied for comparison and several flavonoids were identified; unknown species are being elucidated via LC-MS. Higher levels of foliar flavonoids were induced by UV-B radiation in all species. The two gymnosperms were less affected by the UV-B treatments than the two angiosperms. Q. rubra foliage flavonoid concentrations were more than twice that of any other species, followed by P. tricocarpa, P. menziesii and P. ponderosa. Two-year-old conifer foliage had higher levels of flavonoids than one-year-old tissue. In addition, the composition of the flavonoids shifted as foliage aged. Of the 27 flavonoid peaks found in year-old pine foliage, 18 were higher and 3 were lower in the 2X treatment as compared to the 1X treatment. After two years, only 11 of the flavonoid peaks remained elevated in the 2X treatment, while 7 flavonoids had higher levels in the 1X treatment. Young tissue of P. tricocarpa had higher concentrations of flavonoids than more mature tissue, which also demonstrated reduced treatment effects. |
1Department
of Natural Resource Sciences, Washington State University, Pullman, WA
99164-6410 USA 2Department
of Horticulture and Landscape Architecture, Washington State University,
Pullman, WA 99164-6414 USA 3Department
of Botany, Washington State University, Pullman, WA 99164-4238 USA 4Department
of Rangeland Ecology, University of Idaho, Moscow, ID 83844-1135 USA |